Molecular characterization of fluoroquinolone-resistant Aeromonas spp. isolated from imported shrimp.
نویسندگان
چکیده
Sixty-three nalidixic acid-resistant Aeromonas sp. isolates were obtained from imported shrimp. Phylogenetic analysis of gyrB sequences indicated that 18 were A. enteropelogenes, 26 were A. caviae, and 19 were A. sobria. Double missense mutations in the quinolone resistance-determining region (QRDR) of gyrA at codon 83 (Ser→Val/Ile) and codon 92 (Leu→Met) coupled with a point mutation of parC at codon 80 (Ser→Ile/Phe) conferred high levels of quinolone resistance in the isolates. A majority of A. enteropelogenes and A. caviae strains harbored toxin genes, whereas only a few A. sobria strains harbored these genes. The fluoroquinolone-resistant Aeromonas spp. exhibited higher cytotoxicity than fluoroquinolone-sensitive, virulent Aeromonas spp. to rat epithelial cells.
منابع مشابه
Molecular characterization of the virulent microorganisms along with their drug-resistance traits associated with the export quality frozen shrimps in Bangladesh
Current investigation characterized export quality shrimp samples in terms of pathogenic load along with the drug-resistance traits of the isolates, and detected the major virulent genes present in those isolates. Among the 30 such shrimp samples (15 each of Macrobrachium rosenbergi or Golda and Penaeus monodon or Bagda) studied, almost all were found to be contaminated with a huge load of bact...
متن کاملVirulence and Molecular Characterization of Aeromonas Spp. from Fish Samples Using Ardra Technique
The detection of Virulence factors of Aeromonas spp. is a key component in determining potential pathogenicity because these factors act Multifuntionally and Multifactorially. In this study, totally 50 carp fish samples were collected, Among these, 8 samples were found to be positive for Aeromonas hydrophila and 13 were found to be positive for Aeromonas salmonicida. Antibiotic resistant patter...
متن کاملQuality Evaluation of Some Fresh and Imported Frozen Seafood
This study was done to evaluate the quality parameters of fresh and imported frozen seafood (fillets and shrimp). A total of 120 seafood samples, fillets and shrimp (fresh and imported frozen) 30 each, collected from fish markets at Giza Governorate were sensory, bacteriological, chemical investigated. Panelists rejected 5.0 (16.6%) and 0.0 (0.0%) of fresh fillets and shrimp samples, while the ...
متن کاملMolecular characterization of antibiotic resistance in Pseudomonas and Aeromonas isolates from catfish of the Mekong Delta, Vietnam.
A collection of 116 motile Pseudomonas spp. and 92 Aeromonas spp. isolated from 15 Vietnamese intensive catfish farms was analyzed to examine the molecular antibiotic resistance characteristics and the transferability of resistance markers within and between species. High levels of resistance to ampicillin, trimethoprim/sulfamethoxazole, nalidixic acid, chloramphenicol, and nitrofurantoin were ...
متن کاملMulti-Drug Resistance Mediated by Class 1 Integrons in Aeromonas Isolated from Farmed Freshwater Animals
Aeromonas is regarded as an important pathogen of freshwater animals but little is known about the genetics of its antimicrobial resistance in Chinese aquaculture. The aim of this study was to investigate the presence of integrons and characterize multidrug resistant Aeromonas spp. isolated from diseased farmed freshwater animals. These animal samples included fish, ornamental fish, shrimp, tur...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 78 22 شماره
صفحات -
تاریخ انتشار 2012